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. 2009 Dec 21;206(13):3101–3114. doi: 10.1084/jem.20091925

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© 2009 Schulz et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — R848 administration mobilizes CD103⁺ DC, but not CX3CR1⁺ LPC, into MLN-afferent lymph. (A) Lymph was collected from MLN-afferent vessels 5 h after oral administration of R848 and analyzed by flow cytometry. MHCII⁺CD11c⁻ cells express CD19 and, most likely, represent B cells exiting from GALT (not depicted). Results are representative of six experiments. (B) Frequencies of CD103⁺ DC, CX3CR1^int LPC, and CX3CR1^high LPC 16 h after oral administration of R848 as determined by flow cytometry. CX3CR1^high LPCs were not detected (n.d.) in the MLN. Cell numbers observed in untreated mice were normalized to 1. Bars indicate the according fold change of the mean. Errors bars indicate SD. Four mice per group were analyzed in two independent experiments. Results were obtained with an unpaired Student's t test. **, P < 0.01.