Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec 21;206(13):3089–3100. doi: 10.1084/jem.20091586

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Chorro et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Proliferation of resident epidermal LCs in the steady state. (A) Tiled micrograph of ear epidermal sheets of a 8-wk-old langerin-GFP mouse. LCs are labeled in green and Ki67 is labeled in red. This tile has been obtained by assembling a series of maximal intensity projection Z-stacks. Ki67-expressing LCs are indicated with white circles. (inset) A three-dimensional reconstruction of a Ki67⁺ LC as in D (for more information see Video 2). Results are representative of epidermal sheets from four mice. (B, left) The percentage of Ki67⁺ langerin-GFP⁺ cells at various ages (W4, 4 wk; W8, 8 wk; W14, 14 wk). For each animal, 160–400 cells were analyzed. Means ± SD are displayed (n = 4 mice per experiment). (right) The number of langerin⁺ cells per square millimeter in the ear epidermis of littermate 4-wk-old CCR2^+/+ and CCR2^−/− mice. Means of three experiments ± SD are displayed. (C) Graph shows the distance in micrometers between LCs and their closest neighbor. More than 600 langerin-GFP⁺ cells and >60 Ki67⁺ langerin-GFP⁺ cells were analyzed from the epidermis of 8-wk-old langerin-GFP⁺ mice. Similar results were obtained from the epidermis of 4- and 14-wk-old mice (n = 4). (D) Snapshots of a three-dimensional reconstruction of Z-stacks of 8-wk-old Ki67-expressing epidermal langerin-GFP⁺ cells using Imaris software. Results are representative of epidermal sheets from four mice. For more information, see Video 3 and Video 4. (E) Cell-cycle analysis by flow cytometry of sorted LCs as in Fig. 3 E. Percentages of gated singlet PI⁺ events in the S/G2/M phase are indicated. The experiments have been repeated three times with similar results. (F, top) CD45.1⁺ C57BL/6 recipient mice were irradiated (10 Gy) and injected with10⁷ bone marrow cells obtained from adult langerin-GFP CD45.2⁺ mice to allow identification of donor-derived cells. 5 wk after transplantation, hematopoietic engraftment was analyzed by measurement of blood chimerism, and ears were collected 7 wk after transplantation. (bottom) Micrographs of epidermal sheets of control langerin-GFP mice and of irradiated chimeric mice. Langerin-GFP⁺ cells are labeled in green (anti-eGFP antibody) and langerin⁺ cells are labeled in red (antilangerin antibody). Maximal intensity projections from Z-stacks are displayed. Results are representative of epidermal sheets from four mice. HF, hair follicle; SSC, side scatter.