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. 2009 Dec 21;206(13):3089–3100. doi: 10.1084/jem.20091586

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© 2009 Chorro et al.

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Figure 5. — A keratinocyte-derived signal can drive a massive proliferation of LCs during skin inflammation. (A) Time course of treatment of the ears of mice with MC903 or ethanol. Analysis of the epidermis was performed at days 4 and 14. (B) Graphs show the number of langerin⁺ cells per square millimeter in the epidermis of wild-type mice treated with MC903 (white) or ethanol (black). Means ± SD are displayed (n = 3). (C) Micrographs are representative maximal intensity projections (left) or optical slices (right) of Z-stacks from epidermal sheets of mice treated for 14 d. Langerin⁺ cells are either labeled in red (left) or in green (right), and Ki67 is labeled in red (right). Results are representative of epidermal sheets from two to three animals. The graph shows the percentage of langerin⁺ cells expressing Ki67 after 14 d of MC903 (white) or ethanol (black) treatment. For each animal, 150–300 langerin⁺ cells were analyzed. Means of two to three experiments ± SD are displayed. (D) Similar to the experiment described in Fig. 4 F. 7 wk after transplantation, MC903 or ethanol was applied on the ear every other day for 14 d. Micrographs show epidermal sheets from MC903-treated ears (top) or epidermal sheets from control ethanol-treated ears (bottom). (left) Langerin-GFP⁺ cells are labeled in green (anti-eGFP antibody) and langerin⁺ cells are labeled in red (antilangerin antibody). Maximal intensity projections from Z-stacks are shown. Results are representative of 10 0.56-mm² Z-stacks per mice. The pictures show few (<1%) bone marrow–derived LCs recruited in the epidermis of MC903-treated mice after 2 wk of treatment. (right) Langerin-GFP⁺ cells (green) are not labeled with Ki67 (red). The pictures show two representative examples of langerin-GFP⁺ cells recruited in the epidermis after MC903 treatment. For each animal, >500 Ki67⁺ cells were analyzed. (E) Treatment of VDR^−/− mice with MC903 or ethanol. Mice were treated with MC903 or ethanol as in A, and analysis of the epidermis was performed at day 14. The bar graph represents the percentage of langerin⁺ cells expressing Ki67 in VDR^+/+ or VDR^−/− mice either untreated (gray bar) or treated for 14 d with MC903 (white bar) or ethanol (black bar). For each animal, 250–600 langerin⁺ cells were analyzed. Means of two experiments ± SD are displayed. (F) Similar to the experiment described in E but performed on VDRep^+/+ and VDRep^−/− mice. HF, hair follicle.