Skip to main content
. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Leukemia. 2009 Oct 15;24(1):133–140. doi: 10.1038/leu.2009.192

Table 2.

Immunoglobulin heavy and light chain gene usage in MBL single cells

Familial
MBL
subject		 VH VL
#of
cells
seq.		 V D J Somati
c
Muta-
tionsa
(%)		 Produc
tiveb 		# of
cells
seq.		 V J Somati
c
Muta-
tions
(%)		 Produc
tive
209 36 4-34 3-22 1 N (1.0) P 35 λ2-14 λ3 N (1.7) P
29 κ2-29 κ2 N (0.0) NP
22 4-59 2-8 4 Y (3.6) P 6 κ3-20 κ2 N (1.8) P
6 λ10-54 λ3 N (1.7) P
4 4-34 2-2 4 Y (9.2) P 2 κ1-5 κ1 Y (5.9) P
4 κ4-1 κ1 Y (5.2) NP
0226 c 30 3-15 5-24 4 Y (8.3) P 30 λ1-51 λ2 N (1.1) P
27 κ2-24 κ5 N (0.0) P
3 3-33 3-10 5 Y (6.9) NP 3 λ1-51 λ2 N (1.1) P
3 κ2-24 κ5 N (0.0) P
0504 53 3-23 3-22 3 Y (4.1) P 48 λ3-21 λ3 Y (5.2) P
2 3-7 5-24 4 Y (3.1) P 2 λ4-69 λ3 N (1.1) P
2 κ2-40 κ1 N (0.0) P
2 κ4-1 κ4 N (0.0) NP
0602 35 3-07 3-10 5 Y (3.1) P 29 λ1-51 λ2 N (1.0) P
6 κ4-1 κ4 N (0.0) P
4 3-07 2-21 3 Y (5.9) P 3 κ1-5 κ2 Y (4.5) P
1107 42 3-07 4-17 5 N (1.7) P 35 κ1-33 κ2 Y (3.0) P
31 κ4-1 κ2 Y (3.0) NP
1 3-30 5-5 4 Y (2.4) P 1 κ1-16 κ2 Y (2.3) P
1 3-23 1-26 4 N (0.3) P 1 κ1-5 κ4 N (0.3) P
1109 c 25 3-30 5-5 3 Y (7.0) P 25 λ10-54 λ3 N (1.7) P
8 κ2-29 κ3 N (0.0) NP
7 4-61 2-2 6 Y (3.4) P 5 κ3-20 κ5 Y (3.7) P
5 4-34 2-15 6 Y (9.4) NP 4 κ3-20 κ5 Y (3.7) P
5 4-61 6-13 4 Y (2.4) P 3 κ2-24 κ4 N (0.7) P
4 3-15 5-24 4 Y (2.7) P 4 λ2-14 λ1 Y (2.7) P
3 κ1-33 κ2 N (0.0) NP
3 κ2-30 κ2 N (0.0) NP
2 3-23 2-15 4 Y (8.0) P 1 κ3-11 κ4 N (1.7) P
1 3-7 2-21 1 N (1.3) P 1 κ1-5 κ2 N (0.7) P
a

Percent deviation from germline immunoglobulin sequence. A sequence was designated as mutated if the experimentally obtained immunoglobulin sequence deviated ≥ 2% from germline.

b

P: Immunoglobulin gene rearrangement predicted to be productive; NP: non-productive

c

Single cell analysis from subjects 0226 and 1109 identified single cells with identical light chains but different immunoglobulin heavy chains (subject 0226: VH3-15 and VH3-33; subject 1109: VH4-61 and VH4-34). In both cases one heavy chain rearrangement is predicted to be productive and the other is predicted to be non-productive. As such, these cells are likely derived from a single clone wherein both heavy chain loci are recombined, possibly due to B cell receptor revision events, with one productive rearrangement and the other non-productive. However, since both heavy chains were not amplified from any single cell in either subject, these heavy chain rearrangements are listed separately.