Figure 3. The combination treatment of Δ⁹-THC and CBD specifically inhibits ERK activity.

The effects of cannabinoids on kinases activity were analyzed using Western analysis. In A), U251 cells were treated with vehicle or a combination of Δ⁹-THC (1.7 µM) and CBD (0.4 µM) for three days. Proteins were then extracted and analyzed for pERK, total ERK, pJNK 1/2 and p38 MAPK. In B), U251 cells were treated with Δ⁹-THC (1.7 µM) or CBD (0.4 µM) alone for three days, and analyzed for pERK and total ERK. In C), U251 cells were treated with vehicle or a combination of Δ⁹-THC (1.7 µM) and CBD (0.4 µM) for one and two days. In D), SF126 cells were treated with vehicle or a combination of Δ⁹-THC (1.6 µM) and CBD (1.1 µM) for 12 hours or one day. Either a-tubulin or β-actin was used as a loading control (LC). Blots are representative of at least 3 independent experiments.