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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nano Lett. 2010 Jan;10(1):211–218. doi: 10.1021/nl903295p

Figure 5.

Figure 5

Tracking a quantum dot labeled structure in a live cell in 3D. (A) Fluorescence image of the emitter overlaid on a white light image of the COLO205 cell (scale bar is 2 μm). (B) Images taken of the structure at different points along the trajectory showing different z positions, the bright aggregate at the top edge should be ignored. An average of 2400 photons/frame with a background noise of 8 photons/pixel/frame was measured for this trajectory. (C) Three dimensional plot of the labeled structure's trajectory, showing a variety of diffusive and linear transport characteristics. Points on the curve that correspond to the images in (B) are shown by green shapes.