Fig. 2.

cDNA sequence of the ORF-P 3′ and TLA1 5′ ends. a Overlap between the cDNA sequence of ORF-P and TLA1 is highlighted in gray. Gene-specific primers used for RT-PCR are color coded and underlined. Shown in uppercase bold and underlined fonts are “primer 12F” (blue), “primer 9F” (red), “primer 11F” (green), and “primer 3F” (black). The stop codon TGA of ORF-P and start codon ATG of TLA1 are shown in uppercase bold magenta font. b RT-PCR results using combinations of ORF-P- and TLA1-specific primers. Lane 1 shows 403-bp RT-PCR product obtained with TLA1 forward “primer 11F” and second exon-specific reverse “primer 3R”. Lane 2 shows no RT-PCR result with ORF-P 3′ UTR-specific forward “primer 12F” and TLA1 5′ UTR-specific reverse “primer 11R” (reverse sequence of “primer 11F”). Lane 3 shows 496-bp RT-PCR product obtained with TLA1 5′ UTR-specific forward “primer 9F” and second exon-specific reverse “primer 3R”. Lane 4 shows no RT-PCR result with ORF-P 3′ UTR-specific forward “primer 12F” and TLA1 second exon-specific reverse “primer 3R”. Lane 5 shows 143 bp RT-PCR product obtained with ORF-P 3′ UTR-specific forward “primer 12F” and TLA1 5′ UTR-specific reverse “primer 9R” (reverse sequence of “primer 9F”). F and R stand for forward primer and reverse primer, respectively