Fig. 6.

SDS-PAGE and western blot analysis of Chlamydomonas reinhardtii proteins. Lane 1 tla1-comp1, lane 2 tla1-comp2, lane 3 tla1 mutant, lane 4 wild type. a SDS-PAGE of total cell protein extracts. Lanes were loaded on an equal Chl basis (1.5 nmol Chl per lane). Lane S shows the pre-stained low molecular weight markers. b, c Western blot analysis of C. reinhardtii total cell protein extracts. Lanes, loaded as in a, were probed with TLA1-specific (b) and Lhcb-specific polyclonal antibodies (c). Note the substantially lower levels of the TLA1 and Lhcb proteins in lane 3 (tla1 mutant) relative to that in the other samples