Fig. 3.

Characterization of putative target genes and interacting proteins of MPF1 in Physalis. a Non-lethal β-galactosidase assay for interaction between MPF1, the full-length PFSOC (1–219) and its truncated derivatives (gene and sizes are indicated) in yeast. b, c Bimolecular fluorescence complementation (BiFC) assay after agroinfiltration of MPF1 with PFSOC1 into leaf epidermal cells of P. floridana. Fluorescence (green signal) reveals interaction between MPF1 with PFSOC1 in planta. The relevant combinations of complementation constructs are indicated in the panels. Arrows indicate the nuclei