Fig. 2.

Constructs for RNAi targeting multiple genes in the arabinoxylan feruloyl transferase family PF02458. The pANDA vectors carry a maize Ubiquitin1 promoter and first intron including splicing acceptor sites (Prom. UBI, gray), a nos terminator (T) and the targeted sequences in anti-sense (AS) and sense (S) orientations separated by a gus linker. pAFT-A region I includes a 333-bp segment from gene Os01g42880 (2,438–2,770); region II includes a 332-bp segment from Os04g09590 (6–337). pAFT-B region III includes a 306-bp segment from Os05g08640 (897–1,202); region IV includes a 305-bp segment from Os01g09010 (23–327). Positions are counted from the start codon based on the genomic DNA