Figure 3.
APC upregulates collagen transcripts. A: Cells were exposed for 24 h to 100 nmol/L APC or 1 ng/mL transforming growth factor-β1. Collagen transcripts were measured by real-time reverse transcription-polymerase chain reaction and normalized to those of the RPL0 gene. Results are expressed as fold increase over control values and are the mean ± SE of five experiments; B: Cells were exposed for 6 h to 100 nmol/L APC, in the presence or absence of the mitogen-activated protein kinase (MAPK) inhibitor PD98059 (50 μmol/L), before measuring collagen transcripts. Results are the mean ± SE of three experiments.