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. 2009 Nov 30;187(5):615–622. doi: 10.1083/jcb.200903139

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© 2009 Demonchy et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Characterization of the KIF9A^RNAi mutant. (A and B) IFA on WT (A) or KIF9A^RNAi (B) detergent-extracted trypanosomes. The anti-KIF9A antibody (1:800) labels the flagellum only in noninduced cells. (C and D) Transmission electron micrographs showing cross sections of flagella from WT (C) or 96 h–induced KIF9A^RNAi cells (D). Representative images from 20 sections of WT and 20 sections of KIF9A^RNAi. (E) Western blot on whole cell extracts of control or the indicated T. brucei axoneme mutants (5 × 10⁶ cells/lane) induced for 3 d. The blot was probed with the anti-KIF9A antibody (1:500) and an antialdolase antibody (1:3,000) as a loading control. The experiment was reproduced twice. Values on blots are given in kilodaltons.