Table I.
Analysis of the mitotic parameters in bubR1 mutant neuroblasts
| Straina | Time in colchicine | Mitotic densitybc | Relative mitotic density | Aneuploidyc | PSCSc | Notes |
| min | % | % | ||||
| WT (5) | 0 | 2.11 (0.26) | 1 | 0.2 (0.22)d | NA | viable |
| WT (5) | 30 | 3.36 (0.28) | 1.59 | NA | 0.17 (0.08) | NA |
| WT (5) | 60 | 5.73 (0.19) | 2.72 | NA | 0.20 (0.06) | NA |
| bubR1-KD (5) | 0 | 1.84 (0.12) | 1 | 0.97 (0.14) | NA | viable |
| bubR1-KD (5) | 30 | 2.67 (0.27) | 1.45 | NA | 0.58 (0.21) | NA |
| bubR1-KD (5) | 60 | 4.59 (0.30) | 2.50 | NA | 1.41 (0.72) | NA |
| bubR1-KEN (5) | 0 | 1.64 (0.10) | 1 | 0.67 (0.60) | NA | viable |
| bubR1-KEN (5) | 30 | 1.33 (0.23) | 0.81 | NA | 1.19 (0.47) | NA |
| bubR1-KEN (5) | 60 | 1.25 (0.17) | 0.77 | NA | 3.99 (1.48) | NA |
| bubR1-KEN asp (3) | 0 | 1.46 (0.10) | ND | 4.05 (0.73) | NA | lethal |
| asp (4) | 0 | 7.40 (0.21) | ND | 1.03 (0.26) | NA | lethal |
| bubR1-KD cnn (4) | 0 | 2.92 (0.31) | ND | 1.07 (0.31) | NA | viable |
| bubR1-KEN cnn (5) | 0 | 2.06 (0.20) | ND | 15.22 (6.26) | NA | lethal |
| cnn (3) | 0 | 2.65 (0.24) | ND | 0.83 (0.41) | NA | viable |
| bubR1-KD mad2P (4) | 0 | 1.93 (0.89) | 1 | 12.36 (4.22) | NA | lethal |
| bubR1-KD mad2P (5) | 30 | 1.33 (0.10) | 0.69 | NA | 2.68 (1.98) | NA |
| bubR1-KD mad2P (12) | 60 | 1.20 (0.13) | 0.62 | NA | 4.79 (2.73) | NA |
| bubR1-KEN mad2P (5) | 0 | 1.74 (0.18) | 1 | 0.53 (0.40) | NA | viable |
| bubR1-KEN mad2P (5) | 30 | 1.62 (0.12) | 0.93 | NA | 0.89 (0.17) | NA |
| bubR1-KEN mad2P (4) | 60 | 1.57 (0.12) | 0.91 | NA | 1.58 (0.92) | NA |
| mad2P (5) | 0 | 2.11 (0.26) | 1 | 0.77 (0.37) | NA | viable |
| mad2P (5) | 30 | 1.58 (0.12) | 0.75 | NA | 0.19 (0.18) | NA |
| mad2P (5) | 60 | 1.63 (0.19) | 0.77 | NA | 0.19 (0.20) | NA |
NA, not applicable.
a
The number of brains is shown in parentheses.
b
Mitotic density is defined as the mean number of cells in mitosis per optic field.
c
SD is shown in parentheses.
d
This value was taken from Buffin et al. (2007).