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Overexpression of cfa6 and cfa7. Proteins were labeled with [35S]methionine and [35S]cysteine as described in Experimental Procedures. Constructs were transformed into E. coli BL21(DE3) and overexpressed from the T7 promoter. Lanes: 1, BL21[pBluescript SK(+)]; 2, BL21(pCfa6A); 3, BL21(pCfa6I); and 4, BL21(pCfa7A). Molecular size markers (in kDa) are shown on the left; the migration of Cfa6 and Cfa7 is indicated on the right.
