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. 2009 Nov 23;206(12):2717–2733. doi: 10.1084/jem.20091579

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© 2009 Ceballos et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Capture of HIV-1 by human spermatozoa. Spermatozoa (1.5 × 10⁶/200 µl) were incubated with different amounts of HIV-1 BAL (R5-tropic; A) or HIV-1 IIIB (X4-tropic; B) for 60 min at 37 or 4°C. Cells were then washed thoroughly, lysed, and assayed for p24 antigen by ELISA. Results are the mean ± SEM of six to eight experiments performed in triplicate. In A, spermatozoa was cultured with HIV-1 BAL containing 75 ng p24 for 60 min at 37°C, washed thoroughly, treated with 1,000 U/ml trypsin for 15 min at 37°C, lysed, and assayed for p24 antigen by ELISA. (−) sp represents the values of p24 found in wells in which spermatozoa were omitted (nonspecific attachment of HIV-1 to the wells). The asterisk represents statistical significance (P < 0.05 vs. untreated spermatozoa incubated with 75 ng HIV-1 BAL).