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. 2009 Nov 23;206(12):2685–2699. doi: 10.1084/jem.20092024

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© 2009 The Rockefeller University Press

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Figure 3. — Ets1^−/− MHC I–restricted maturelike DP thymocytes migrate to the medulla. (A) Thymocytes from Ets1^+/+ and Ets1^−/− mice were stained for surface expression of CD4, CD8, TCRβ and the chemokine receptor CCR7. Overlaid histograms (right plots) analyze expression of CCR7 on Ets1^−/− DP and CD4 SP thymocytes (bottom graph), and on Ets1^+/+ CD8 and CD4 SP thymocytes (top graph), all TCRβ^hi (as gated in left plots). Expression of CCR7 on TCR^lo DP thymocytes is shown in both strains as a negative control (dark gray histograms). Data are representative of two experiments. (B) Frozen thymic sections were prepared from P14 transgenic Ets1^+/+ or Ets1^−/− mice, stained for cytokeratin 14 (K14, pseudo-colored as blue, defining medullary areas), CD4 (red), and CD8 (green). Overlaying CD4 and CD8 staining (right) shows exclusion of DP cells from medullary areas in Ets1^+/+ but not in Ets1^−/− mice. The red medullary staining in Ets1^+/+ mice is contributed by the few CD4 SP cells that develop in these recombination-competent animals. Bars, 100 µm. Data are representative of three experiments.