Figure 4.

Ets1^−/− MHC I–restricted maturelike DP thymocytes are CD8 lineage cells. Expression of the genes encoding Thpok (Zbtb7b) and perforin (Prf1) was analyzed by real-time RT-PCR on sorted Vα2^hi CD24^lo CD4⁺CD8⁺ and CD4⁻CD8⁺ thymocyte populations from Ets1^+/+ and Ets1^−/− mice carrying the P14 TCR transgene (left three columns) and on TCR^hi CD4⁺CD8⁻ thymocytes from wild-type mice (right column). mRNA levels, normalized on β-actin expression in the same sample, are shown relative to those in wild-type CD4⁺CD8⁻ cells (Thpok) or Ets1^+/+ P14 CD4⁻CD8⁺ cells (Prf1). Bars indicate the mean values derived from triplicate determination from a single sorted population; error bars show standard deviations. Data are representative of three or more independent sorted samples for each population.