Table II.
Clones with IgM+ and IgG+ members in human PB memory B cells
| Donor | VH-gene analyzed | CD27+ B cells | CDRIII primersa | Products with monoclonal CDRIIIb | Clones with IgM and IgG membersc |
| % | (%) | (%) | |||
| 1 | VH6 | 30 | 15 | 5 (33) | 2 (40) |
| 2 | VH6 | 40 | 20 | 2 (10) | 0 |
| 3 | VH6 | 45 | 20 | 3 (15) | 0 |
| 4 | VH6 | 30 | 15 | 3 (20) | 3 (100) |
| 5 | VH6 | 25 | 8 | 4 (38) | 2 (50) |
| 6 | VH6 | 19 | 8 | 4 (50) | 4 (100) |
| 7 | VH3-23 | 29 | 30 | 9 (30) | 1 (11) |
| 8 | VH3-23 | 21 | 30 | 7 (23) | 2 (29) |
a
Number of CDRIII-primers which amplified a specific product from diluted DNA of the respective IgG+ B cell clone.
b
Counted are only those products which gave rise to a sequence with distinct CDRIII, i.e. those where the CDRIII-primer specified a unique clone.
c
The number of clones with IgM+ and IgG+ B cells. The frequency of clones among monoclonal products is shown in brackets.