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GFP-actin remodeling in a subconfluent EC layer treated with cytochalasin D at t=0:00. White arrows indicate filament shortening, edge retraction, or speckle formation. Green arrows follow centripetal motion of actin aggregate that was formed at a leading edge. (A) No-flow control after addition of cytochalasin D. (B) After onset of flow at t=0:00 with medium containing cytochalasin D. Time=mm:ss. Bar=10 μm.
