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Cell area (mean ± SD) relative to t=0 min indicated by GFP-actin in (A) subconfluent and (B) confluent EC layers. At t=0 min, either cytochalasin was added under no-flow conditions (open circles) or simultaneously with the onset of steady unidirectional flow (closed circles) at a wall shear stress of 15 dyn/cm2. *Significantly different than no-flow (p<0.05).
