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Capillary depletion with or without vascular washout. The majority of PrPc was in the parenchymal space, demonstrating that PrPc was not sequestered by the capillary bed but crossed the full thickness of the capillary wall. Washout produced no difference in parenchymal values, showing that PrPc was not reversibly retained by the luminal surface of the capillary bed.
