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. 2009 Dec 29;107(1):58–63. doi: 10.1073/pnas.0900121106

Fig. 1.

Fig. 1.

Repression of Ras-induced senescence by Myc in REFs depends on Ser-62. (A) Primary REFs were transfected with expression plasmids for Myc and/or oncogenic Ras. The micrographs show transfected cells cultured for 5 days before analysis of senescence by SA-β-Gal staining. (B) Mean values and SDs of SA-β-Gal staining (blue columns) of at least three experiments are given based on analysis of 50 randomly chosen cells. Apoptosis (red columns) was analyzed as described in Materials and Methods. (C–F) REFs were transfected and analyzed as in A and B using activated c-Raf or MEK or were treated with TPA (C and D) or transfected with different Myc mutants (E and F). (G) Focus formation assay of REFs transfected with Ras + WT Myc or mutants. (H) Number of foci in G was counted (blue columns) and analyzed for SA-β-Gal staining (red columns) 12 days after transfection. Contr, control.