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. 2009 Dec 29;107(1):58–63. doi: 10.1073/pnas.0900121106

Fig. 3.

Fig. 3.

IFN-γ abrogates repression of senescence in v-Myc-expressing human U-937 cells, correlating with induced p27 expression, inhibited Cdk2 activity, and reduced Ser-62 phosphorylation. (A) Schematic picture describing the U-937 differentiation model. IFN-γ reverses the v-Myc block of monocytic differentiation and senescence. (B) U-937-GTB parental cells (Left) and U-937-myc-6 cells (Right) were treated with TPA and/or IFN-γ for 6 days before SA-β-Gal staining. Contr, control. (C) Mean values and SDs of SA-β-Gal staining are given based on analysis of 50 randomly chosen cells. Ser-62 phosphorylation (D) and p27 expression (F) of IFN-γ + TPA-treated cells as determined by immunoblot analysis. (E) Cell lysates immunoprecipitated with cyclin E antibodies were assayed in vitro for histone H1 phosphorylation. Rosc, roscovitine.