Table 1.
New microsatellite markers and primers developed for this study.
| Marker name | Genetic (Cytogenetic) location | Forward primer (5' – 3') | Repeat type | Reverse primer (5' – 3') | Sequenced allele size (bp) |
| Chromosome 2 | |||||
| H682 | (2R, 7B) | CGGGCGGTCGTTATTAGGAG | (AC)7 | CTGAGTTGACACGAGTTGCC | 73 |
| 3F11-C1 | (2R,9B) | AGATGGTGTCCTTCGGTGTC | (GT)17 | GTGGAGGAGAAGCCACACAT | 156 |
| 3B20-B1 | (2L, 24B) | GAGACCAAAACATGTCAACCG | (CA)17 | GTATGTCTGCGCATGAACGAT | 122 |
| R007 | 71.1 (2L, 26D) | GCACCAGTCAAATACGCACT | (CA)3+9 | GTCCGTGTTGTTCGAAGATG | 184 |
| Chromosome 3 | |||||
| H249 | 11.9 (3R, 30A) | GGAAGCAGCTGCCTTTTATG | (TG)15 | ACAATGTAACACAGCGCGAG | 161 |
| 3A13-12 | (3R, 30E) | GAACAAAACGTGCCACAGC | (GT)9 | TCCCCGACTTACGTGAACC | 168 |
| 3A7-37 | (3R, 31B) | CGATTGGAACCAACAAGTGG | (CA)2+9 | AAGCCATCTCTTGACGTTCG | 151 |
| Ddc* | (3R, 33B) | CGTTGAGAACCGAAAACAGG | (GT)10 | ATCGGGACACTCAGCAACAG | 121 |
| 3A6-17 | (3R, 33C) | TGTGAAGAATGTTTTCGCTGAC | (AC)17 | CTCCCCCGTGTAATTTGAAC | 120 |
| 9D17-42 | (3R, 33C) | TAAACCTCACCGTCCACATC | (TG)23+5 | AAATTGAAACCCGTCCAAAG | 150 |
| 6B10-1 | (3R, 33D) | CATGAGTGCCGCTGTAAATG | (TG)2+16 | ATCGCTCTCCTCCTCTAGCC | 120 |
| 3A23-100 | (3R, 34A) | AATGGACCATCGTTAGCACAG | (TG)12 | TCCAAAGTTTTCCCTTCCAC | 136 |
| 3L09-C1 | (3L, 38C) | GAAACTTCCCTGAATTTCCGC | (CA)10+8 | ATGGAGGCGACTAAACGTTGC | 169 |
| 3A2-29 | (3L, 41A) | GGCTGCTCTAGGAGGATGTG | (CT)16 | AATCGAGGGTGGTGTGAATG | 189 |
| 3O10-C2 | (3L, 41D) | TTAATCACCCACCTTGATCGC | (CA)18 | CCTAGAGCTTGGAATCGAATC | 101 |
| 3A14-11 | (3L, 43D) | GTAATAGCGAGCGAGCGAAC | (TG)36 | GAGCGATCCGAATCGTTAGT | 180 |
| 3A14-47 | (3L, 43D) | GGAGGCACTCTACCACAACC | (GT)10 | CCGAACGTCATCGCTAATG | 104 |
| 3A19-32 | (3L, 43D) | CGTGCTGGCATTAAAAAGTG | (CA)15 | CTACGCATGCATCTTTCTCC | 138 |
| 3A5-25 | (3L, 45A) | CCTTGGGCGCTATAAAACAC | (TG)10 | TATTAGCAGCAGGGGAAACC | 131 |
| 19A18-54 | (3L, 46A) | TAATGCAGTGGTGCGGTTC | (GT)5+6 | AATCACAGCCCAGTCAGTCC | 121 |
| 19C20-B1 | (3L, 46B) | ACCAAGTGCTCTTTGGGTTG | (TG)15 | ACGAATGCTGGACAATGTGA | 141 |
The cytogenetic location of H682 is A. gambiae scaffold predicted by it's location in AAAB01008987, contig 566 (GenBank accession AAAB01008987). New primer pairs were designed for markers R007 [44] and H249 [14]. Sequenced allele size is expressed in base pairs (bp). * This Dopa decarboxylase microsatellite is contained within the large first intron of predicted Ddc isoform 1. DNA sequence is from G3 strain (GenBank # AF063021; P. Romans, unpublished).