Skip to main content
. 2009 Dec 29;107(1):109–114. doi: 10.1073/pnas.0906121107

Fig. 5.

Fig. 5.

Characterization of somatic tetraploid RGCs in the P1 chick retina. SBC in DiI retrograde-labeled RGCs (A–C, E, and F) or flow cytometry (D). (A) Estimation of the soma areas of diploid (2C) and tetraploid (4C) RGCs. Dots represent the mean obtained in random retinal regions. Lines represent the range of sizes observed in all of the regions analyzed. (B) Correlation between soma sizes from diploid (2C) versus tetraploid (4C) RGCs in random retinal areas, as estimated from (A). (C) Distribution of dendritic field sizes from diploid (2C) or tetraploid (4C) RGCs as evidenced from SBC. (D) DNA content (DAPI) in dissociated retinal cells double-immunostained with anti-Islet1 and anti-β2AChR antibodies. Most Islet1/β2AChR-double labeled cells were observed to be tetraploid (4C). (E) Simultaneous analysis of DNA content (DAPI) in either total or β2AChR-positive cells located in the GCL demonstrates that cells expressing β2AChR are mostly tetraploid. A few events with DNA content above 4C can be observed (asterisk), likely due to doublets of 2C and 4C cells. (F) A representative plot showing the distribution of soma sizes in the β2AChR-positive cell population located in the GCL.