Fig. 4.

Silencing the expression of endogenous NCLX decreases mitochondrial Ca²⁺ efflux that can be rescued by expression of recombinant murine NCLX. (A) Mitochondrial Ca²⁺ responses following application of ATP (40 μM, as in Fig. 3A) was measured in HEK-293 cells cotransfected with either the siNCLX or a scrambled siRNA construct (si control) and the RP-mt–expressing plasmid. Averaged rates of mitochondrial Ca²⁺ efflux are shown (Right) (n = 9, **P < 0.01). (B) The same experiment as in A was performed on SHSY-5Y cells transfected with NCLX shRNA plasmid alone or together with the murine NCLX-encoding plasmid (which is insensitive to the NCLX shRNA construct). Averaged rates of mitochondrial Ca²⁺ efflux are shown (Right) (n = 11, *P < 0.05).