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. 2009 Dec 29;107(1):169–174. doi: 10.1073/pnas.0907739107

Fig. 4.

Fig. 4.

Long-lived E(z) and esc mutants have reduced levels of H3K27me3 that are partially restored by mutations in trx. (A) Quantitative Western blots from age-matched E(z)63 and esc9 adult male heterozygotes out-crossed to C-S. E(z)63/+ and esc9/+ mutants have, respectively, 35% less and 45% less H3K27me3 than their respective sibling controls and C-S controls. (B) Quantitative Western blots from age-matched E(z)60/+, trxB11/+, and E(z)60 trxB11/+ + adults out-crossed to C-S. E(z)60/+ mutants have 35% less H3K27me3 than C-S. trxB11/+ mutants have 13% more H3K27me3 than C-S. E(z)60 trxB11/+ + double mutants have 14% more H3K27me3 than E(z)60/+ mutants. The C-S control value was arbitrarily set to 1 in each experimental replicate. The normalized means from three independent replicates of each genotype are plotted with SEM (error bars). t-tests were performed to test for statistical significance. * indicates P ≤ 0.05 compared to C-S. + indicates P ≤ 0.05 compared to E(z)60/+.