Skip to main content
. 2009 Dec 29;107(1):52–57. doi: 10.1073/pnas.0909153107

Fig. 2.

Fig. 2.

Phosphorylation of Ser157 of p45 by active JNK. (A) Interaction of the endogenous p45 with P-JNK. WCE were prepared from MEL cells with or without anisomycin treatment (5 μg/mL for 30 min), IP with anti-p45, anti-P-JNK, or the preimmune serum, and then analyzed by WB with anti-p45, anti-P-JNK, and anti-actin antibodies. CL, WCE without IP. (B) In vitro phosphorylation of p45 by P-JNK. Myc-tagged GST-p45-derived proteins (as indicated) were incubated with the active JNK in the in vitro phosphorylation reactions and analyzed either by WB and autoradiography. (C) Effects of S157A and S346A mutations on P-JNK-induced degradation of p45. CB3 cell pools stably expressing the indicated Myc-tagged GST-p45 variant were treated with 60 μM MG132 for 1 h, 5 μg/mL anisomycin for 2 h, or a combination of both. The WCE were then analyzed by WB with anti-Myc and anti-tubulin antibodies.