Fig. 5.

Kinetics of changes of the expression levels and chromatin binding of p45 and Bach1 during the early phase of MEL differentiation. (A) Changes of expression. The MEL cells were harvested at 12, 15, 18, 21, and 24 h after DMSO induction and analyzed by Northern blotting and WB. The relative levels of the mRNAs and proteins were calculated and compared in the histogram. (B) Changes of chromatin binding. ChIP assay was used to examine the differential bindings of p45 and Bach1 at the HS2 in MEL cells at differential time of DMSO induction. The signals were detected by quantitative RT-PCR and standardized to those of the input: the preimmune samples (pre) and the β-actin gene. The relative intensities of the PCR signals and folds of increases over the 0-h sample were then calculated.