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Incubation-time dependent degradation of aRNA. 5 μg of total RNA were amplified and labeled (either with Cy3-UTP or Cy5-UTP) with the T7-System for 2, 4, 6, 8 and 16 hours. The aRNA was separated on a denaturing agarose gel and photographed on a gel-imaging system. Fainter bands in the Cy5-labeled aRNA are due to quenching of Cy5-fluorescence by SybrGreen II and lower yield. M=Molecular weight marker.
