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Incubation of aRNA in the absence of T7 RNA Polymerase. Cy3- or Cy5-labeled aRNA that had been synthesized by the optimal '4 hour protocol' was incubated under amplification conditions (37°C, amplification buffer) in the absence of T7 RNA Polymerase for 2, 8 and 16 hours. The aRNA was separated on a denaturing agarose gel and photographed on a gel-imaging system. M=Molecular weight marker. The slightly weaker band in Cy5 (8 h) might be due to small loading differences.
