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. 2009 Dec 29;107(1):430–435. doi: 10.1073/pnas.0909468107

Fig. 5.

Fig. 5.

Effect of myosin phosphorylation on the fluorescence during the chase phase and on the amplitude of the slow exponential decay. (A) The effect of myosin phosphorylation on the fluorescence intensity in the chase phase: 7% phosphorylation (□), 85% phosphorylation (○). (B) The amplitude of the slow exponential decay during the chase phase is shown as a function of the level of phosphorylation of the myosin regulatory light chain. The temperature was 24°C. Error bars show the SEM for 10 to 20 different runs. The solid line in (B) is an interpolation between the points. Fits to the data in (A) are defined as follows: 7% phosphorylation, P1 = 0.64 ± 0.01, T1 = 14 ± 1 s, P2 = 0.28 ± 0.01, T2 = 230 ± 40 s; 85% phosphorylation, P1 = 0.73 ± 0.01, T1 = 11 ± 1 s, P2 = 0.22 ± 0.01, T2 = 125 ± 6 s.