Table 1.

Parameters of the two exponential fit to the fluorescence intensity during the chase phase

Incubationa	Chase	P1c	T1 (sec)	P2	T2 (sec)	nd
mantATP	ATP	0.65 ± 0.04	20 ± 2	0.31 ± 0.01	230 ± 24	24
ATP	mantATP	0.58 ± 0.05	19 ± 3	0.33 ± 0.02	246 ± 25	8
mantATPb	ATP	0.63 ± 0.02	17 ± 3	0.32 ± 0.02	156 ± 20	5
mantATP	ADP	0.96 ± 0.02	17 ± 5	0.02 ± 0.01	60 ± 240e	7
mantADP	ATP	0.91 ± 0.01	14 ± 6	0.06 ± 0.01	276 ± 180	5
mantATP +ATP	ATP	0.92 ± 0.01	11 ± 3	0.04 ± 0.01	222 ± 80	5

^aThe first two columns give the nucleotides in the initial incubation and the chase solutions.

^bThis row gives the averages for soleus fibers, all other rows were done with psoas fibers.

^cP1 and P2 are the magnitudes and T1 and T2 are the lifetimes for the first and second phases of the two exponential function that was fit to the data.

^dErrors are standard errors of the mean for n experiments.

^eFor some measurements, the second component was too small to be well defined, and the errors in the lifetime were large.