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. 2009 Dec 29;107(1):442–447. doi: 10.1073/pnas.0911790107

Fig. 2.

Fig. 2.

Activity of recombinant dfsAC. (A) Coomassie blue-stained SDS/PAGE gel of His-tagged dfsAC (53 KDa expected) purified from E. coli. (B) dfsAC activity assayed at a constant pH of 7.75 in the presence of 2.5 mM ATP, 20 mM MgCl2, 0.5 mM MnCl2, and the indicated concentrations of NaHCO3. EC50 = 4.9 mM NaHCO3. (C) dfsAC activity at the indicated pH. (D) dfsAC activity in the presence of 2.5 mM ATP, 5 mM MnCl2, and the indicated concentrations of KH7 or 4CE. IC50 for KH7 = 9.6 μM and IC50 for 4CE = 46.9 μM. Values are expressed as fold stimulation relative to the baseline in each experiment (0 mM HCO3−, pH 7.75, or no drug, respectively). Data points reflect the averages of four independent assays for each condition.