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. 2009 Dec 29;107(1):442–447. doi: 10.1073/pnas.0911790107

Fig. 5.

Fig. 5.

sAC-dependent VHA translocation during localized alkalosis in isolated gill. (A) Percentage of cells showing VHA translocation in gill fragments internally perfused and incubated in alkaline saline with or without 100 μM 4CE (n = 5). A repeated-measures two-way ANOVA (P < 0.05 for treatment, time, and interaction) or the Tukey–Kramer planned least square means test was used. Letters indicate different levels of statistical significance. Bicarb, bicarbonate. Representative VHA immunostaining of gills internally perfused and incubated in alkaline saline and DMSO (B) or 4CE (C).