Fig. 5.

PTCH1 repression is alleviated by 5-aza-2′-deoxycytidine treatment. Reverse transcriptase PCR (RT-PCR) analysis is shown of PTCH1 isoforms, AXIN2, and PRDM8 from the human medulloblastoma cell line UW228 and normal cerebellum. UW228 was treated with either 5 μM 5-aza-2′-deoxycytidine (5-aza-dC) or vehicle alone (DMSO, Mock) for 72 h and RNA was analyzed by RT-PCR (SI Materials and Methods). −, no RT control. A schematic of the PTCH1 promoter region with alternative splicing events and CpG islands 1 (analyzed in Fig. 4C) and 2 (evaluated in ref. 28) is depicted (adapted from ref. 42).