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. 2009 Dec 29;107(1):282–287. doi: 10.1073/pnas.0912373107

Fig. 2.

Fig. 2.

Effect of mutagenesis of the gE N-terminal region on gE/IDE and gE/gI interactions. Analysis of gE/IDE (A) and gE/gI (B) interactions in melanoma cells infected with the gE N-terminal mutants. Analysis of gE/IDE (C) and gE/gI (D) interactions in rOka-ΔP27-G90 and rOka-ΔY51-G90-infected melanoma cells. L, lysate; IP, immunoprecipitation; C, IP control. (E) Coimmunoprecipitation of gE and IDE in Hek293 cells transfected with the expression plasmids carrying the wt gE, gE ΔP27-Y51, gE ΔY51-G90, or gE ΔP27-G90. gE was immunoprecipitated with MAb anti-gE (Chemicon) (A and E) or MAb 3B3 (B and C), whereas gI was immunoprecipitated with MAb anti-gI 6B5 (D). gI was detected with rabbit anti-gI v67 (B), IDE with rabbit anti-IDE antibody (A, C, and E), and gE with MAb 3B3 (D). Ui, uninfected cells lysates; vector, lysates from cells transfected with the empty vector.