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. 2009 Dec 29;107(1):282–287. doi: 10.1073/pnas.0912373107

Fig. 5.

Fig. 5.

Effect of mutagenesis of gE aa51-187 region on VZV entry and replication in human T cells. (A) Coimmunoprecipitation of gE-ΔY51-P187 and IDE in transfected Hek293 cells. gE was immunoprecipitated with rabbit anti-HA; MAb anti-IDE was used to detect IDE. L, lysate; IP, immunoprecipitation; C, IP control; vector, lysates from cells transfected with the empty vector. (B) Human T cells from uninfected thymus/liver xenografts were infected by coculturing with rOka- or rOka-ΔY51-P187-infected HELF for 30 h. The T cells were stained with anti-CD3 antibody and anti-VZV immune serum and analyzed by flow cytometry. The error bars indicate SD. The titer of the infected monolayers is indicated in the table. *, P = 0.049. Number of replicates, n = 2. (C) Thymus/liver xenografts were inoculated with HELF infected with rOka, rOka-Y51, rOka-S31A, or rOka-ΔY51-P187. The number of samples from which infectious virus was recovered per total xenografts inoculated is on the horizontal axis. Each bar represents the mean titer for those yielding virus ± SE (D) Thymus/liver xenografts inoculated with rOka- or rOka-ΔP27-G90-infected HELF. Each bar represents the mean titer ± SD for samples yielding virus and containing > 5% CD3+ cells. The titers of the inoculum are shown as pfu/mL.