Table 1.

Efficiency of I-SceI–mediated transgenesis of MyHC::mCherry

	Total injected MyHC::mCherry	%
Injected zygotes	1,610	100
24-h survival	1,442	90
24-h expression	582	40
Recovered primary polyps	539	34
Expressing primary polyps	120	7.5
Germline transmission*		8–40

*Germline transmission is given as the percentage of primary polyps with transgenic patches, which varies greatly because of the mosaicism of the transgenic germline.