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. 1998 Dec 22;95(26):15481–15486. doi: 10.1073/pnas.95.26.15481

Figure 3.

Figure 3

The effect of λRexB on the E. coli mazEF programmed cell death induced by ppGpp (A) and by amino acid starvation with serine hydroxamate (B). (A) The experiment includes E. coli strains MC4100, MC4100(λ), MC4100(λrexB∷Ω), MC4100/pLDG1 (carrying λrexB), MC4100/pRS14 (carrying λrexBUAA), MC4100 clpP∷cat (SG22098), and MC4100ΔmazEF. ppGpp induction was carried out by using truncated relA gene as described in Materials and Methods. (B) The experiments included the relA+ derivatives of E. coli strain MC4100, MC4100(λ), MC4100ΔmazEF, MC4100(λrexB∷Ω), MC4100/pLDG1 (carrying λrexB), and MC4100/pRS14 (carrying λrexBUAA). We included MC4100relA− as a control. Amino acid starvation was carried out by treating the cells with serine hydroxamate as described in Materials and Methods. In A and B, cell survival was measured by colony-forming ability, and the data presented are the mean of at least five independent experiments.