Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Description or relevant genotype | Source or reference |
|---|---|---|
| E. coli strain | ||
| MC4100 | araD139 Δ(argF-lac)205 flbB5301 ptsF25 rpsL150 deoC1 relA1 | 24 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE relA1 lac [F′ proAB lacIqZΔM15 Tn10(TetR)] | Stratagene |
| JC7623(λ) | recBCsbcBC(λ) | 25; A. Cohen |
| MC4100lacIq | By conjugation from XL1-Blue | This study |
| SG22098 | clpP∷cat in MC4100 background | 13; S. Gottesman |
| MC4100ΔmazEF | A ΔmazEF derivative of MC4100 | 20 |
| MC4100(λ) | MC4100 lysogenized with λ | This study |
| MC4100(λrexB∷Ω) | MC4100 lysogenized with λrexB∷Ω | This study |
| MC4100(λΔBamΔH1) | MC4100 lysogenized with λΔBamΔH1 | 20 |
| MC4100relA+ | A relA+ derivative of MC4100 | This study |
| MC4100relA+ΔmazEF | A relA+ derivative of MC4100ΔmazEF | This study |
| MC4100relA+(λ) | MC4100relA+ lysogenized with λ | This study |
| MC4100relA+(λrexB∷Ω) | MC4100relA+ lysogenized with λrexB∷Ω | This study |
| Plasmids | ||
| pGB2 | pSC101-based vector, SpcR/SmR | 26 |
| pGB2ts | pGB2 derivative, thermo-sensitive for replication; SpcR/SmR | 27; M. Yarmolinsky |
| pGB2ts∷phd-doc | Derivative of pGB2ts expressing phd and doc; SpcR/SmR | 18; M. Yarmolinsky |
| pHAL20 | pKK233-3 derivative carrying phd-doc under ptac; AmpR | M. Yarmolinsky |
| pALS13 | pBR322 derivative carrying a truncated relA gene under ptac and a lacIq; AmpR | 28, 29 |
| pRLM74 | pRLM74 carries the 1.5kb AluI fragment of λ which carries λrexB; AmpR | 4; M. McMacken |
| pRS2UAG | pKC30 derivative carrying a UAG mutation in λrexB at position 36231-36233 of λ DNA (31); AmpR | 4 |
| pRS2UAA | pKC30 derivative carrying a UAA mutation in λrexB at position 36231-36233 of λ DNA (31); AmpR | 4 |
| pRS10 | pBR322 derivative carrying λrexB under the pLJT promoter; AmpR, λrexB was subcloned from pRLM74 | This study |
| pRS11 | pBR322 carrying λrexBUAG subcloned from pRS2UAG; AmpR | This study |
| pRS12 | pBR322 carrying λrexBUAA subcloned from pRS2UAA; AmpR | This study |
| pLDG1 | pSU-2718 derivative carrying λrexB under the pLJT promoter; CmR | 4 |
| pLGD1∷Ω | pLGD1 carrying the omega interposon in the λrexB gene | This study |
| pRS14 | pSU-2718 carrying λrexBUAA subcloned from pRS2UAA; CmR | This study |
| pRS15 | pGB2 carrying λrexB under pLJT; SpcR/StrR; λrexB was subcloned from pRLM74 | This study |
| pRSE | pBR322 derivative carrying mazE under λpL; AmpR | 20 |
| pRSE1 | pRSE carrying λrexB under pLJT promoter; AmpR; λrexB was subcloned from pRLM74 | This study |
| pAAC | An E. coli-Streptomyces shuttle vector carrying the omega interposen aacC4 gene confering gentamicin resistance | 30 |
| pHG335 | pKK233-3 derivative carrying λcII under ptac and lacIq; AmpR | A. Oppenheim |