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. Author manuscript; available in PMC: 2010 Jan 14.
Published in final edited form as: J Immunol. 2007 Jun 1;178(11):7199–7210. doi: 10.4049/jimmunol.178.11.7199

FIGURE 7.

FIGURE 7

Endolysosomal tubules colocalize with polymerizing microtubules, and nocodazole disrupts tubules in class II MHC-eGFP-expressing DCs. A, BMDCs were transduced to express YFP-tubulin and CD63-mRFP1. After LPS stimulation, cells that formed endolysosomal tubules were imaged as shown. B, Administration of 10 µM nocodazole to class II MHC-eGFP tubulating cells; serial images at the times indicated are shown. Nocodazole administration was arbitrarily assigned the value of 0 s (t = 0′00″). C, LPS-stimulated BMDCs expressing CD63-mRFP1 and EB1-eGFP were imaged. CD63+ tubules are in close apposition to EB1 “comets.” D, Over time, the CD63 tubules (long arrow) followed the EB1 path (short arrows).