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. 2010 Jan 14;5(1):e8703. doi: 10.1371/journal.pone.0008703

Figure 3. Genotypic and protein characterization of Hras −/−,Nras −/− and Kras −/− fibroblasts by PCR and Western blot.

Figure 3

(A) DNA PCR of K-ras −/− fibroblasts cultures; K-ras −/− fibroblasts are identified by a 270-bp product, and wild type fibroblasts by a 360-bp product (see methods section); mRNA (B) and protein (C) expression of Ras isoforms in K-ras −/− fibroblasts cultures; mRNA expression of H, N, K-Ras4A and K-Ras4B isoforms, evaluated by PCR (D), and protein expression (E) of H, N, and K-Ras isoforms, evaluated by Western blot, in Hras −/−,Nras −/− fibroblasts. K-Ras (1): mouse anti-human K-Ras (H3845-M01) from Abnova; K-Ras (2): mouse anti-mouse K-Ras (R3400) from Sigma.