Figure 6. K-Ras expression analysis in nuclear and cytoplasmic fractions of human mesangial cells and K-ras ^−/− fibroblasts by western blot.

K-Ras (1): mouse anti-human K-Ras (H3845-M01) from Abnova; K-Ras (2): mouse anti-mouse K-Ras (R3400) from Sigma; K-Ras (3): mouse anti-human K-Ras (sc-522) from Santa Cruz Biotechnologies. Cyclin A, histone H1 and tubulin were used as purity controls of nuclear (cyclin A, histone H1) and cytoplasmic fractions (tubulin), respectively.