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. 2010 Jan 14;5(1):e8715. doi: 10.1371/journal.pone.0008715

Figure 2. Deletion of proline-902 exposes a functional PDZ motif in GluA4 and confers binding to SAP97.

Figure 2

(A) Expression of wild-type or mutant GluA4, with or without co-expressed myc-tagged SAP97 in HEK293 cells. Upper panels show expression of all proteins; lower panels show co-immunoprecipitation of GluA4ΔP, but not full-length GluA4 with SAP97. Immunoblotting antibodies are indicated on right. (B) Transiently expressed GluA4ΔP can co- immunoprecipitate with endogenous SAP97 from HEK293 cells. Upper panel shows similar expression levels of transfected GFP-tagged constructs. Lower panel show immunoprecipitation with anti-SAP97 specific antibody. Both blots were probed with anti-GFP IgG. The extreme carboxyterminal sequences of the expressed proteins are shown below.