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(A) Total numbers of hit proteins detected in eluates off each affinity column. (B) Known substrates for Rho-kinase detected in eluates off each affinity column. The numbers indicate protein scores by Mascot analysis. (C) The numbers of proteins specifically or commonly detected in GST-Rho-kinase-cat and GST-PKN-cat columns are shown. These results are representatives of at least three independent experiments.
