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. 2009 Dec 9;3(1-2):73–83. doi: 10.1242/dmm.003293

Fig. 4.

Fig. 4.

KRIT1 protein is expressed widely and regulates β-catenin signaling in epithelial cells. (A) KRIT1 protein was quantified based on a standard curve of recombinant human glutathione S-transferase (GST)-KRIT1 FERM (protein 4.1, ezrin, radixin, moesin) domain protein. Data are expressed as nanograms of human protein equivalents per milligram of total cell protein, ± two standard errors (S.E.). Cell types: BAEC, bovine endothelial; FLS, mouse primary synoviocyte; RAW, mouse macrophage cell line; Jurkat, human T-cell line; SMC, mouse primary aortic smooth muscle cells; MCF10 (MCF10A), normal human mammary epithelial cell line; CHO, Chinese hamster ovary epithelial cell line. (B) β-Catenin reporter activity in MDCK cells after KRIT1 depletion (black bars) and in the presence of constitutively active Rap1 (white bars). TOPFlash activity is given as in Fig. 1C, P<0.0001 by ANOVA, *P<0.05 by Bonferroni post-hoc test compared with negative control siRNA-transfected cells, **P<0.05 compared with β-catenin expressing cells, n=4.