Skip to main content
. 2010 Jan 15;6(1):e1000817. doi: 10.1371/journal.pgen.1000817

Figure 2. Requirement of ETG1 for establishment of sister chromatid arm cohesion.

Figure 2

(A) Percentage of positional separation frequencies per homologous chromosome (number of investigated nuclei in parentheses) analyzed in wild-type (Col-0) and etg1-1 mutant plants after FISH with the labeled T2P11 or T7N9 BACs from chromosome 1. The FISH probe pAL detects the centromeric 178-bp repeats. (B–D) Structural arrangement of FISH signal positions in 4C nuclei counterstained with DAPI. (B) Positional alignment (T2P11) at both chromosome 1 homologs. Two of 10 centromeric signals associated (arrow). (C) Positional sister chromatid separation at both homologs. (D) Positional association of both homologs. (E) Percentage of sister chromatid alignment/separation frequencies analyzed in wild-type (Col-0) and etg1-1. (F) Identical frequencies of centromere-specific FISH signals in wild-type and etg1-1 nuclei.