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. 2010 Jan 15;6(1):e1000817. doi: 10.1371/journal.pgen.1000817

Figure 6. Inefficient DNA repair upon loss of sister chromatid cohesion.

Figure 6

(A) Real-time RT-PCR analysis of DNA stress-inducible genes PARP2, BRCA, and CYCB1;1 in wild-type (Col-0), etg1-1, wild-type (Nos-0), ctf18-1, and etg1-1 ctf18-1 plants. Total RNA prepared from 8-day-old seedlings was amplified by RT-PCR. All values were normalized against the expression level of the ACTIN2 gene. (B) Statistical analysis of a comet assay. The average %-values of DNA in tails of nuclei of 7-day-old wild-type (Col-0), etg1-1, wild-type (Nos-0), ctf18-1, and etg1-1 ctf18-1 seedlings. Error bars indicate SD. (C) Examples of comets from plant nuclei with undamaged (top) or damaged (bottom) DNA. (D) Kinetics of DSB repair in wild-type versus etg1-1 mutant plants. Fractions of remaining DSB were calculated for 0, 5, 10, 20, and 60 min recovery time after treatment with 50 µg/ml bleomycin for 1 h. Maximum damage was normalized as 100% at t = 0. (E–G) Wild-type (Col-0, left) and etg1-1 (right) plants were grown on medium holding 50 ppm methyl methane sulfonate (E), 3 µg/ml mitomycin C (F), or no supplement (G). Plants were photographed 3 weeks after sowing.