Table 2. Combinations used in the sub-colony experiment evaluating whether the presence/absence of antagonism impacts the success of ant fungiculture.

		Presence of antagonism (Mean ZOI in cm; n = 3)
Colony of origin of ants and Pseudonocardia	Colony-of-origin of cultivar	Degree of antagonism from cultivar	Degree of antagonism from Pseudonocardia (no head start)	Degree of antagonism from Pseudonocardia (with head start)	Direction of Conflict*
A	K	0	2.0	0	None
	D	0.20	1.4	0	Cultivar
	A (control)	0	2.0	0.40	Bacterium
C	J	0.10	2.2	0	Cultivar
	D	0.10	2.4	0	Cultivar
	C (control)	0	2.2	0	None
D	E	0	1.8	0	None
	J	0.10	2.6	0	Cultivar
	D (control)	0.10	2.0	0	Cultivar
C	I	0.10	2.2	0.60	Both
	D	0.10	2.4	0	Cultivar
	C (control)	0	2.2	0	None
H	I	0.10	1.6	0.63	Both
	K	0	1.4	0	None
	H (control)	0.30	1.9	0	Cultivar
A	I	0	2.1	0.60	Bacterium
	D	0.20	1.4	0	Cultivar
	A (control)	0	2.0	0.40	Bacterium
F	I	0.20	1.3	0	Cultivar
	D	0.33	1.7	0.50	Both
	F (control)	0.70	1.6	0.57	Both

*Based on the Pseudonocardia-cultivar inhibition bioassay where the cultivar was given a head start, since the vast majority of pairings in the former bioassay (no head start) displayed some inhibition.

The column on the far right indicates the direction of antagonism, from either cultivar towards Pseudonocardia (cultivar), from Pseudonocardia towards cultivar (bacterium), or from neither (none) or both (both) of the symbionts. See Table 1 for species and geographical origin of colonies.